Affiliation:
1. U.S. Food and Drug Administration, National Center for Toxicological Research, Office of Research, Division of Chemistry, Jefferson, AR 72079
Abstract
Abstract
A reversed-phase (ODS-2) liquid chromatographic method was developed to determine low nanogramper-gram levels of sulfadiazine (SDZ) in salmon muscle tissue. SDZ was extracted with acetonitrile-aqueous 2% acetic acid (pH 3.0), partitioned into methylene chloride, and cleaned up by using a strong-cation-exchange, solid-phase extraction cartridge. SDZ was derivatized postcolumn with fluo-rescamine and detected by fluorescence. The limit of detection was 0.2 ng SDZ/g tissue. Recoveries from coho salmon tissue fortified with 1,5,10, and 20 ng SDZ/g tissue averaged 84.5,85.0,83.6, and 83.9%, respectively; recoveries from Atlantic salmon tissue fortified with 10 ng SDZ/g tissue averaged 82.6%.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
12 articles.
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