Affiliation:
1. Eli Lilly and Company, Lilly Research Laboratories, 2001 W Main St, Greenfield, IN 46140-0708
Abstract
Abstract
A method is described for detection and quantitation of monensin in bovine tissues by liquid chromatography (LC) with postcolumn derivatization (PCD) with vanillin. Monensin is extracted from the tissues by homogenization with methanol–water and is isolated and concentrated by liquid–liquid partition and sorbent extraction with silica gel. Monensin is mixed postcolumn with vanillin under acidic conditions and heated, and the resulting products are measured by a variable-wavelength detector at 520 nm. The method has a limit of quantitation of 5 ppb monensin in milk and 25 ppb monensin in bovine muscle, liver, kidney, and fat. Standard recovery over the levels and matrixes tested ranged from 80 to 88%. The method is an improvement in specificity, accuracy, and analysis time over existing monensin residue methods for bovine tissues.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
9 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献