Aurora B promotes the CENP-T–CENP-W interaction to guide accurate chromosome segregation in mitosis-Reference-Cited by-同舟云学术

Aurora B promotes the CENP-T–CENP-W interaction to guide accurate chromosome segregation in mitosis

Published:2024-01-10 Issue:2 Volume:16 Page:
ISSN:1674-2788
Container-title:Journal of Molecular Cell Biology
language:en
Short-container-title:

Author:

Liu Wei¹²^ORCID,Dou Zhen¹³^ORCID,Wang Chunyue¹,Zhao Gangyin¹,Wu Fengge¹,Wang Chunli⁴,Aikhionbare Felix²,Ye Mingliang⁴^ORCID,Sedzro Divine Mensah¹,Yang Zhenye¹,Fu Chuanhai¹,Wang Zhikai¹²³,Gao Xinjiao¹,Yao Xuebiao¹³,Song Xiaoyu¹³,Liu Xing¹³^ORCID

Affiliation:

1. MOE Key Laboratory for Cellular Dynamics, Center for Advanced Interdisciplinary Science and Biomedicine of IHM, University of Science and Technology of China School of Life Sciences , Hefei 230027 , China

2. Keck Center for Cellular Dynamics and Organoids Plasticity , Atlanta, GA 30310 , USA

3. Anhui Key Laboratory for Cellular Dynamics and Chemical Biology, Hefei National Research Center for Interdisciplinary Sciences at the Microscale , Hefei 230026 , China

4. National Chromatographic Research and Analysis Center, Chinese Academy of Sciences , Dalian 116023 , China

Abstract

Abstract Accurate chromosome segregation in mitosis depends on kinetochores that connect centromeric chromatin to spindle microtubules. Centromeres are captured by individual microtubules via a kinetochore constitutive centromere-associated network (CCAN) during chromosome segregation. CCAN contains 16 subunits, including CENP-W and CENP-T. However, the molecular recognition and mitotic regulation of the CCAN assembly remain elusive. Here, we revealed that CENP-W binds to the histone fold domain and an uncharacterized N-terminal region of CENP-T. Aurora B phosphorylates CENP-W at threonine 60, which enhances the interaction between CENP-W and CENP-T to ensure robust metaphase chromosome alignment and accurate chromosome segregation in mitosis. These findings delineate a conserved signaling cascade that integrates protein phosphorylation with CCAN integrity for the maintenance of genomic stability.

Funder

National Key Research and Development Program of China

Publisher

Oxford University Press (OUP)

Link

https://academic.oup.com/jmcb/advance-article-pdf/doi/10.1093/jmcb/mjae001/55403775/mjae001.pdf

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