DNA/RNA helicase DHX36 is required for late stages of spermatogenesis-Reference-Cited by-同舟云学术

DNA/RNA helicase DHX36 is required for late stages of spermatogenesis

Published:2022-12-09 Issue: Volume: Page:
ISSN:1674-2788
Container-title:Journal of Molecular Cell Biology
language:en
Short-container-title:

Author:

Zhang Kejia¹,Zhang Tianxin¹,Zhang Yujie¹,Yuan Jinyu¹,Tang Xinzhe¹,Zhang Chaobao¹,Yin Qianqian¹,Zhang Yonglian¹,Tong Ming-Han²¹

Affiliation:

1. State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, University of Chinese Academy of Sciences, Chinese Academy of Sciences , Shanghai 200031 , China

2. School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Chinese Academy of Sciences , Hangzhou 310024 , China

Abstract

Abstract Spermatogenesis is a highly complex developmental process that typically consists of mitosis, meiosis, and spermiogenesis. DNA/RNA helicase DHX36, a unique guanine-quadruplex (G4) resolvase, plays crucial roles in a variety of biological processes. We previously showed that DHX36 is highly expressed in male germ cells with the highest level in zygotene spermatocytes. Here, we deleted Dhx36 in advanced germ cells with Stra8-GFPCre and found that a Dhx36 deficiency in the differentiated spermatogonia leads to meiotic defects and abnormal spermiogenesis. These defects in late stages of spermatogenesis arise from dysregulated transcription of G4-harboring genes, which are required for meiosis. Thus, this study reveals that Dhx36 plays crucial roles in late stages of spermatogenesis.

Publisher

Oxford University Press (OUP)

Subject

Cell Biology,Genetics,Molecular Biology,General Medicine

Link

https://academic.oup.com/jmcb/advance-article-pdf/doi/10.1093/jmcb/mjac069/47762700/mjac069.pdf