Comprehensive autophagy evaluation in cardiac disease models

Author:

Kaludercic Nina1ORCID,Maiuri Maria Chiara2,Kaushik Susmita3,Fernández Álvaro F4ORCID,de Bruijn Jenny5ORCID,Castoldi Francesca2ORCID,Chen Yun6,Ito Jumpei7ORCID,Mukai Risa8ORCID,Murakawa Tomokazu7,Nah Jihoon8ORCID,Pietrocola Federico9ORCID,Saito Toshiro10ORCID,Sebti Salwa4ORCID,Semenzato Martina1112,Tsansizi Lorenza1112ORCID,Sciarretta Sebastiano1314,Madrigal-Matute Julio3ORCID

Affiliation:

1. Neuroscience Institute, Department of Biomedical Sciences, National Research Council of Italy (CNR), 35131, Padova, Italy

2. Centre de Recherche des Cordeliers, INSERM, Sorbonne Université, Université Paris Descartes, Université Paris Diderot, 75006, Paris, France

3. Department of Developmental and Molecular Biology, Institute for Aging Studies, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA

4. Center for Autophagy Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA

5. Department of Pathology, Cardiovascular Research Institute (CARIM), Maastricht University, P. Debyelaan 25, 6229 HX, Maastricht, The Netherlands; Institute of Molecular Cardiovascular Research (IMCAR), RWTH Aachen, University, Pauwelsstrase 30, 52074, Aachen, Germany

6. Departments of Medicine (Cardiology) and Cell Biology, Wilf Family Cardiovascular Research Institute, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY, 10461, USA

7. The School of Cardiovascular Medicine and Sciences, King's College London British Heart Foundation Centre of Excellence, London SE5 9NU, UK

8. Department of Cell Biology and Molecular Medicine, Cardiovascular Research Institute, Rutgers New Jersey Medical School, 185 South Orange Avenue, Newark, NY, USA

9. Cellular Plasticity and Disease Laboratory. Institute for Research in Biomedicine (IRB Barcelona), Barcelona; Institute of Science and Technology (BIST), Barcelona, Spain

10. Department of Surgery and Clinical Science, Graduate School of Medicine, Yamaguchi University, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505, Japan

11. Department of Biology, University of Padua, Via U Bassi 58B, 35121, Padua, Italy

12. Venetian Institute of Molecular Medicine, Via Orus 2, 35129, Padua, Italy

13. Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100, Latina, LT, Italy

14. Department of AngioCardioNeurology, IRCCS Neuromed, 86077, Pozzilli, IS, Italy

Abstract

Abstract Autophagy is a highly conserved recycling mechanism essential for maintaining cellular homeostasis. The pathophysiological role of autophagy has been explored since its discovery 50 years ago, but interest in autophagy has grown exponentially over the last years. Many researchers around the globe have found that autophagy is a critical pathway involved in the pathogenesis of cardiac diseases. Several groups have created novel and powerful tools for gaining deeper insights into the role of autophagy in the aetiology and development of pathologies affecting the heart. Here, we discuss how established and emerging methods to study autophagy can be used to unravel the precise function of this central recycling mechanism in the cardiac system.

Funder

Fondation Leducq Transatlantic Network of Excellence

American Heart Association

Italian Ministry of Health

Publisher

Oxford University Press (OUP)

Subject

Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology

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