Affiliation:
1. Department of Chemistry, Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Indonesia , Depok 16424 , Indonesia
Abstract
Abstract
An electrochemical sensor of SARS-CoV-2 spike glycoprotein S1 has been developed using N-acetyl neuraminic acid (Neu5Ac) as the biosensing agent based on the interaction between Neu5Ac with the N-terminal domain of spike glycoprotein S1. Gold-modified screen-printed carbon electrodes (Au-SPCEs), prepared by electrochemical deposition of gold particles on a screen-printed carbon electrode surface, were used to detect the presence of the SARS-CoV-2 from the change in the electrochemical behavior of Neu5Ac. Cyclic voltammetry of Neu5Ac in phosphate buffer solution pH 7.6 using Au-SPCEs yielded a couple of oxidation and reduction peaks at potentials of +0.6 and 0.1 V (vs Ag/AgCl), respectively. The currents of both peaks linearly increased with the Neu5Ac concentrations in the range from 0 to 200 µM. A sensitivity of 0.77 mA/mM and 0.47 mA/mM for reduction and oxidation currents were observed, respectively, with estimated detection limits (LODs) of 51.05 µM and 35.77 µM. In the presence of SARS-CoV-2 spike glycoprotein S1, these current peaks decreased. At an optimum contact time of 30 min, a linear correlation to the current change of the reduction peaks was observed for the spike glycoprotein S1 over the concentration range 0 to 0.01 µg/mL. An estimated LOD of 0.7 ng/mL could be achieved with excellent stability (2.18% RSD value for n = 10), indicating that the sensor is promising for use in real applications of SARS-CoV-2 spike glycoprotein S1 detection.
Funder
Hibah PUTI Q1 Universitas Indonesia 2022
Publisher
Oxford University Press (OUP)
Cited by
2 articles.
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