Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections-Reference-Cited by-同舟云学术

Performance of TEM-PCR vs Culture for Bacterial Identification in Pediatric Musculoskeletal Infections

Published:2018-05-22 Issue:6 Volume:5 Page:
ISSN:2328-8957
Container-title:Open Forum Infectious Diseases
language:en
Short-container-title:

Author:

Wood James B¹²,Sesler Cheryl³,Stalons Donald³,Grigorenko Elena³,Schoenecker Jonathan G⁴⁵⁶,Creech C Buddy⁷,Thomsen Isaac P⁷

Affiliation:

1. Division of Pediatric Infectious Diseases and Global Health, Department of Pediatrics, Indianapolis, Indiana

2. Division of Pediatric and Adolescent Comparative Effectiveness Research, Indiana University School of Medicine, Indianapolis, Indiana

3. Diatherix-Eurofins, Huntsville, Alabama

4. Department of Orthopaedics, Vanderbilt University School of Medicine, Nashville, Tennessee

5. Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee

6. Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee

7. Division of Pediatric Infectious Diseases, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee

Abstract

Abstract Improved diagnostics are needed for children with musculoskeletal infections (MSKIs). We assessed the performance of target-enriched multiplex polymerase chain reaction (TEM-PCR) in children with MSKI. TEM-PCR was concordant with culture in pathogen identification and antibiotic susceptibility testing, while increasing the overall yield of pathogen detection. This technology has the potential to inform judicious antimicrobial use early in the disease course.

Funder

National Institutes of Health

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Oncology

Link

http://academic.oup.com/ofid/article-pdf/5/6/ofy119/33575830/ofy119.pdf

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