Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides-Reference-Cited by-同舟云学术

Improving the Sensitivity of Real-time PCR Detection of Group B Streptococcus Using Consensus Sequence-Derived Oligonucleotides

Published:2018-07-01 Issue:7 Volume:5 Page:
ISSN:2328-8957
Container-title:Open Forum Infectious Diseases
language:en
Short-container-title:

Author:

Khatami Ameneh¹,Randis Tara M¹,Chamby Anna¹,Hooven Thomas A²,Gegick Margaret¹,Suzman Evan¹,A’Hearn-Thomas Brady³,Steenhoff Andrew P⁴⁵,Ratner Adam J¹

Affiliation:

1. Departments of Pediatrics and Microbiology, New York University School of Medicine, New York, New York

2. Department of Pediatrics, Columbia University College of Physicians and Surgeons, New York, New York

3. Department of Pediatrics, University of Iowa Carver College of Medicine, Iowa City, Iowa

4. Department of Pediatrics, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania

5. Division of Pediatric Infectious Diseases, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania

Abstract

Abstract Group B Streptococcus (GBS) is a perinatal pathogen and an emerging cause of disease in adults. Culture-independent GBS detection relies on polymerase chain reaction (PCR) of conserved genes, including sip. We demonstrate suboptimal sensitivity of the existing sip PCR strategy and validate an improved method based on consensus sequences from >100 GBS genomes.

Funder

Doris Duke Charitable Foundation

National Institutes of Health

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Oncology

Link

http://academic.oup.com/ofid/article-pdf/5/7/ofy164/33591536/ofy164.pdf

Reference14 articles.

1. Group B streptococcal infections;Randis;Pediatr Rev,2017

2. Prevention of perinatal group B streptococcal disease—revised guidelines from CDC, 2010;Verani;MMWR Recomm Rep,2010