Affiliation:
1. Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC) , Gif-sur-Yvette 91198, France
2. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Hokkaido Center , Sapporo 062-8517, Japan
3. Institute for Agro-Environmental Sciences, National Agriculture and Food Research Organization (NARO) , Tsukuba 305-8604, Japan
Abstract
Abstract
Caballeronia insecticola is a bacterium belonging to the Burkholderia genus sensu lato, which is able to colonize multiple environments like soils and the gut of the bean bug Riptortus pedestris. We constructed a saturated Himar1 mariner transposon library and revealed by transposon-sequencing that 498 protein-coding genes constitute the essential genome of Caballeronia insecticola for growth in free-living conditions. By comparing essential gene sets of Caballeronia insecticola and seven related Burkholderia s.l. strains, only 120 common genes were identified, indicating that a large part of the essential genome is strain-specific. In order to reproduce specific nutritional conditions that are present in the gut of Riptortus pedestris, we grew the mutant library in minimal media supplemented with candidate gut nutrients and identified several condition-dependent fitness-defect genes by transposon-sequencing. To validate the robustness of the approach, insertion mutants in six fitness genes were constructed and their growth deficiency in media supplemented with the corresponding nutrient was confirmed. The mutants were further tested for their efficiency in Riptortus pedestris gut colonization, confirming that gluconeogenic carbon sources, taurine and inositol, are nutrients consumed by the symbiont in the gut. Thus, our study provides insights about specific contributions provided by the insect host to the bacterial symbiont.
Funder
JSPS Research Fellowship for Young Scientists, Japan
Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan
Ministry of Higher Education, Research, and Innovation, France
CNRS International Research Project, France
JSPS-CNRS Bilateral Open Partnership Joint Research Project, France-Japan
Agence Nationale de la Recherche, France
Saclay Plant Sciences-SPS
Publisher
Oxford University Press (OUP)
Cited by
1 articles.
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