Scanning Electron Microscopy and Geometric Contour Morphometry for Identifying Eggs of Three Amazonian Species of Mansonia (Diptera: Culicidae)-Reference-Cited by-同舟云学术

Scanning Electron Microscopy and Geometric Contour Morphometry for Identifying Eggs of Three Amazonian Species of Mansonia (Diptera: Culicidae)

Published:2019-12-10 Issue:3 Volume:57 Page:745-754
ISSN:0022-2585
Container-title:Journal of Medical Entomology
language:en
Short-container-title:

Author:

da Silva Ferreira Francisco Augusto¹^ORCID,Simões Rejane de Castro²,Ferreira-Keppler Ruth Leila³,Alencar Jeronimo⁴,Scarpassa Vera Margarete⁵,Tadei Wanderli Pedro⁶

Affiliation:

1. Programa de Pós-Graduação em Entomologia – PPGEnt - Instituto Nacional de Pesquisas da Amazônia – INPA; Avenida André Araújo, 2.936 – CEP: 69067–375 – Manaus, Amazonas, Brazil

2. Programa de Pós-Graduação em Biotecnologia – PPGBIOTEC - Universidade Federal do Amazonas – UFAM; Av. General Rodrigo Octavio Jordão Ramos, 1200 – CEP: 69067-005 - Manaus – Amazonas, Brazil

3. Laboratório de Taxonomia e Sistemática de Insetos Aquáticos – Instituto Nacional de Pesquisas da Amazônia – INPA; Avenida André Araújo, 2.936 – CEP: 69067–375 – Manaus, Amazonas, Brazil

4. Laboratório de Diptera - Instituto Oswaldo Cruz – FIOCRUZ; Avenida Brasil 4365, CEP: 21040–360, Manguinhos – Rio de Janeiro, Brazil

5. Laboratório de Genética de Populações e Evolução de Mosquitos Vetores – Coordenação de Biodiversidade (COBIO) - Instituto Nacional de Pesquisas da Amazônia – INPA; Avenida André Araújo, 2.936 – CEP: 69067–375 – Manaus, Amazonas, Brazil

6. Laboratório de Malária e Dengue – Coordenação de Sociedade, Ambiente e Saúde (COSAS) - Instituto Nacional de Pesquisas da Amazônia – INPA; Avenida André Araújo, 2.936 – CEP: 69067–375 – Manaus, Amazonas, Brazil

Abstract

Abstract The eggs of three Amazonian species of the genus Mansonia (Diptera: Culicidae) were analyzed using morphological and morphometric characters. Eggs of Mansonia humeralis Dyar & Knab, 1916 were morphologically different from those of Mansonia titillans (Walker, 1848) and Mansonia amazonensis (Theobald, 1901), which were more similar to each other according to linear and geometric morphometry. A principal component analysis generated from elliptic Fourier contour data (PC1—92.6% and PC2—2.61%) indicated that Ma. amazonensis and Ma. titillans are more similar to each other than either is to Ma. humeralis. Discriminant multivariate analysis was highly accurate with only four classification errors and a 90% accuracy rate. The results indicate that the three Amazonian species can be precisely distinguished in the egg stage and that geometric morphometry based on elliptic Fourier contours is a promising method for distinguishing eggs of species of Mansonia. An identification key based on egg morphology is provided to distinguish the four Neotropical species.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Insect Science,General Veterinary,Parasitology

Link

http://academic.oup.com/jme/article-pdf/57/3/745/33161833/tjz240.pdf

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