Macroevolutionary variation and environmental correlates of scalation traits in Eurasian vipers (Serpentes: Viperinae)

Abstract

Abstract Understanding how phenotypic variation across species is shaped by the combination of shared evolutionary history and environmental factors is key to elucidating the processes that underlie biodiversity. In reptiles, morphological traits have traditionally been used to delimit species and make systematic inferences. Recent studies highlight the possibility that phenotypic variation, particularly in scalation traits, might instead be driven by environmental factors and therefore not reflect the phylogenetic relationships among species. In this study, we combined morphological and ecological data in a macroevolutionary framework, in order to describe the morphological variation across species of Eurasian vipers (Serpentes: Viperinae), investigate the phylogenetic structure of scalation traits and test the contribution of environmental factors in shaping morphological patterns. We found considerable variation in all examined traits, which, in most cases, agreed with the phylogenetic relationships among species, reinforcing their usefulness for taxonomic inferences. Interestingly, however, the number of ventral scales exhibited lower phylogenetic signal and a tight association with environmental factors of geographical ranges, suggesting potential adaptive or developmental sources of variation in the trait. This is the first comparative study of macroevolutionary variation in scalation traits in Eurasian vipers, validating the use of most of them for systematic inferences, but also indicating possible environmental factors that might shape phenotypic variation across species.