Patterns of morphological differentiation within Manacus manacus (Aves: Pipridae) in Colombia: revisiting hypotheses of isolation and secondary contact

Abstract

Abstract Determining the factors responsible for phenotypic variation within species is a fundamental task in evolutionary ecology. Isolation by distance, isolation by environment and clines along secondary contact zones between formerly isolated populations are common patterns of morphological variation. In this study, we evaluated whether patterns of morphological variation exhibit association with isolation by distance, isolation by environment or secondary contact in populations of Manacus manacus with contrasting plumage colour. We used field and museum measurements of five morphological characters from 311 individuals from 88 localities distributed across a highly heterogeneous environmental range within Colombia. Climatic variables explained a higher proportion of the variation than geographic distance among localities. We found differences in wing and culmen length between white and yellow phenotypes of M. manacus in Colombia. Overall morphology did not change in a clinal fashion in relation to the contact zone and the few traits that exhibit clinal variation suggest a distant locality for the contact zone, not in line with our expectations by secondary contact between differentiated populations, suggesting alternative mechanisms for differentiation between subspecies. Our results suggest that this species exhibits marked morphological variation associated with changes in temperature and precipitation. These associations are consistent throughout the geographic range analysed, leading us to postulate that dispersal and local adaptation jointly shape the distribution of phenotypic variation.