SeedTransNet: a directional translational network revealing regulatory patterns during seed maturation and germination

Author:

Bai Bing12ORCID,Schiffthaler Bastian3ORCID,van der Horst Sjors4ORCID,Willems Leo2ORCID,Vergara Alexander3,Karlström Jacob1,Mähler Niklas1ORCID,Delhomme Nicolas3ORCID,Bentsink Leónie2ORCID,Hanson Johannes1ORCID

Affiliation:

1. Umeå Plant Science Center, Department of Plant Physiology, Umeå University , SE-901 87 Umeå , Sweden

2. Wageningen Seed Science Centre, Laboratory of Plant Physiology, Wageningen University , 6708 PB Wageningen , The Netherlands

3. Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences , Umeå , Sweden

4. Department of Molecular Plant Physiology, Utrecht University , 3584 CH Utrecht , The Netherlands

Abstract

Abstract Seed maturation is the developmental process that prepares the embryo for the desiccated waiting period before germination. It is associated with a series of physiological changes leading to the establishment of seed dormancy, seed longevity, and desiccation tolerance. We studied translational changes during seed maturation and observed a gradual reduction in global translation during seed maturation. Transcriptome and translatome profiling revealed specific reduction in the translation of thousands of genes. By including previously published data on germination and seedling establishment, a regulatory network based on polysome occupancy data was constructed: SeedTransNet. Network analysis predicted translational regulatory pathways involving hundreds of genes with distinct functions. The network identified specific transcript sequence features suggesting separate translational regulatory circuits. The network revealed several seed maturation-associated genes as central nodes, and this was confirmed by specific seed phenotypes of the respective mutants. One of the regulators identified, an AWPM19 family protein, PM19-Like1 (PM19L1), was shown to regulate seed dormancy and longevity. This putative RNA-binding protein also affects the translational regulation of its target mRNA, as identified by SeedTransNet. Our data show the usefulness of SeedTransNet in identifying regulatory pathways during seed phase transitions.

Funder

Netherlands Organization for Scientific Research

Swedish government

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Physiology

Reference76 articles.

1. Deciphering transcriptional and metabolic networks associated with lysine metabolism during Arabidopsis seed development;Angelovici;Plant Physiology,2009

2. Extensive translational regulation during seed germination revealed by polysomal profiling;Bai;New Phytologist,2017

3. Seed-stored mRNAs that are specifically associated to monosomes are translationally regulated during germination;Bai;Plant Physiology,2020

4. MEME SUITE: tools for motif discovery and searching;Bailey;Nucleic Acids Research,2009

5. Analyses of reactive oxygen species and antioxidants in relation to seed longevity and germination;Bailly;Methods in Molecular Biology,2011

Cited by 4 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3