The transcriptional co-regulators NBCL1 and NBCL2 redundantly coordinate aerial organ development and root nodule identity in legumes

Author:

Liu Shengbin12ORCID,Magne Kévin12ORCID,Zhou Jing3ORCID,Laude Juliette12ORCID,Dalmais Marion12ORCID,Le Signor Christine4ORCID,Bendahmane Abdelhafid12ORCID,Thompson Richard4,Couzigou Jean-Malo3ORCID,Ratet Pascal12ORCID

Affiliation:

1. Université Paris-Saclay, CNRS, INRAE, Univ Evry, Institute of Plant Sciences Paris-Saclay (IPS2) , 91190, Gif sur Yvette , France

2. Institute of Plant Sciences Paris-Saclay (IPS2), Université de Paris , 91190 , Gif sur Yvette , France

3. Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS, Toulouse INP , 31320, Auzeville Tolosane , France

4. Agroécologie, AgroSup Dijon, Institut National de la Recherche Agronomique (INRAE), Université Bourgogne Franche-Comté , 21000, Dijon , France

Abstract

Abstract Medicago truncatula NODULE ROOT1 (MtNOOT1) and Pisum sativum COCHLEATA1 (PsCOCH1) are orthologous genes belonging to the NOOT-BOP-COCH-LIKE (NBCL) gene family which encodes key transcriptional co-regulators of plant development. In Mtnoot1 and Pscoch1 mutants, the development of stipules, flowers, and symbiotic nodules is altered. MtNOOT2 and PsCOCH2 represent the single paralogues of MtNOOT1 and PsCOCH1, respectively. In M. truncatula, MtNOOT1 and MtNOOT2 are both required for the establishment and maintenance of symbiotic nodule identity. In legumes, the role of NBCL2 in above-ground development is not known. To better understand the roles of NBCL genes in legumes, we used M. truncatula and P. sativum nbcl mutants, isolated a knockout mutant for the PsCOCH2 locus and generated Pscoch1coch2 double mutants in P. sativum. Our work shows that single Mtnoot2 and Pscoch2 mutants develop wild-type stipules, flowers, and symbiotic nodules. However, the number of flowers was increased and the pods and seeds were smaller compared to the wild type. Furthermore, in comparison to the corresponding nbcl1 single mutants, both the M. truncatula and P. sativum nbcl double mutants show a drastic alteration in stipule, inflorescence, flower, and nodule development. Remarkably, in both M. truncatula and P. sativum nbcl double mutants, stipules are transformed into a range of aberrant leaf-like structures.

Funder

China Scholarship Council (CSC) PhD scholarship

Centre National de la Recherche Scientifique

French Laboratory of Excellence (Labex) Saclay Plant Sciences

French National Research Agency under the program ‘Investissements d’avenir’

French Laboratory of Excellence (Labex) project ‘TULIP’

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Physiology

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