Affiliation:
1. National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, 300 Fenglin Road, Shanghai 200032, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
Abstract
Abstract
The cytochrome b6f (cyt b6f) acts as a common linker of electron transport between photosystems I and II in oxygenic photosynthesis. PetM, one of eight subunits of the cyt b6f complex, is a small hydrophobic subunit at the outside periphery, the functional mechanism of which remains to be elucidated in higher plants. In this work, we found that unlike the PetM mutant in Synechocystis sp. PCC 6803, the Arabidopsis thaliana PetM mutant showed a bleached phenotype with yellowish leaves, block of photosynthetic electron transport and loss of photo-autotrophy, similar to the Arabidopsis PetC mutant. Although PetM is relatively conserved between higher plants and cyanobacteria, Synechocystis PetM could not rescue the PetM-knockout phenotype in Arabidopsis. We provide evidence that the Synechocystis PetM did not stably bind to the Arabidopsis cyt b6f complex. Based on these results, we suggest that PetM is required by Arabidopsis to maintain the function of the cyt b6f complex, likely through its close link with core subunits to form a tight ‘fence’ that stabilizes the core of the complex.
Funder
Strategic Priority Research Program of CAS
Funding for Synthesis Biology
National Key Grant for Transgenic Crop
National Natural Science Foundation of China
Publisher
Oxford University Press (OUP)
Subject
Cell Biology,Plant Science,Physiology,General Medicine
Cited by
4 articles.
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