Improved design and analysis of CRISPR knockout screens

Author:

Chen Chen-Hao123,Xiao Tengfei14,Xu Han125,Jiang Peng12,Meyer Clifford A12,Li Wei1267ORCID,Brown Myles14,Liu X Shirley12

Affiliation:

1. Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, MA, USA

2. Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute and Harvard School of Public Health, Boston, MA, USA

3. Biological and Biomedical Science Program, Harvard Medical School, Boston, MA, USA

4. Department of Medical Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA, USA

5. Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, TX, USA

6. Center for Genetic Medicine Research, Children’s National Health System, Washington, DC, USA

7. Department of Genomics and Precision Medicine, The George Washington School of Medicine and Health Sciences, Washington, DC, USA

Funder

NIH

Breast Cancer Research Foundation

BCRF

National Institutes of Health

Center for Genetic Medicine Research

Gilbert Family Neurofibromatosis Institute at Children’s National Health System

Publisher

Oxford University Press (OUP)

Subject

Computational Mathematics,Computational Theory and Mathematics,Computer Science Applications,Molecular Biology,Biochemistry,Statistics and Probability

Reference41 articles.

1. Genomic copy number dictates a gene-independent cell response to CRISPR-Cas9 targeting;Aguirre;Cancer Discov.,2016

2. BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis;Canver;Nature,2015

3. Multiplex genome engineering using CRISPR/Cas systems;Cong;Science,2013

4. Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome;DeKelver;Genome Res.,2010

5. A new class of temporarily phenotypic enhancers identified by CRISPR/Cas9-mediated genetic screening;Diao;Genome Res.,2016

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