Affiliation:
1. Laboratory of Transcription Dynamics, Research Center for Biological Visualization, Institute for Quantitative Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan
2. Applied Mathematics Department, University of Granada, Granada, Spain
3. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan
Abstract
Abstract
Regulatory interactions between enhancers and core promoters are fundamental for the temporal and spatial specificity of gene expression in development. The central role of core promoters is to initiate productive transcription in response to enhancer's activation cues. However, it has not been systematically assessed how individual core promoter elements affect the induction of transcriptional bursting by enhancers. Here, we provide evidence that each core promoter element differentially modulates functional parameters of transcriptional bursting in developing Drosophila embryos. Quantitative live imaging analysis revealed that the timing and the continuity of burst induction are common regulatory steps on which core promoter elements impact. We further show that the upstream TATA also affects the burst amplitude. On the other hand, Inr, MTE and DPE mainly contribute to the regulation of the burst frequency. Genome editing analysis of the pair-rule gene fushi tarazu revealed that the endogenous TATA and DPE are both essential for its correct expression and function during the establishment of body segments in early embryos. We suggest that core promoter elements serve as a key regulatory module in converting enhancer activity into transcription dynamics during animal development.
Funder
Grant-in-Aid for Transformative Research Areas
Grant-in-Aid for Scientific Research on Innovative Areas
Grant-in-Aid for Scientific Research
Grant-in-Aid for Challenging Research
Grant-in-Aid for Research Activity Start-up
Japan Society for the Promotion of Science
Ministry of Education, Culture, Sports, Science and Technology
Tomizawa Jun-ichi & Keiko Fund of Molecular Biology Society of Japan for Young Scientist
Mochida Memorial Foundation for Medical and Pharmaceutical Research
Nakajima Foundation
Inamori Foundation
Takeda Science Foundation
Sumitomo Foundation
Senri Life Science Foundation
Mitsubishi Foundation
Grant-in-Aid for Early-Career Scientists
Employment Stability Support for Young Researchers from the University of Tokyo
JST
FPI
MINECO-Feder
University of Granada
Publisher
Oxford University Press (OUP)
Cited by
15 articles.
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