Occurrence of Klebsiella pneumoniae ST244 and ST11 extensively drug-resistant producing KPC, NDM, OXA-370 in wastewater, Brazil

Author:

Montenegro Kaylanne12,Flores Claudia3ORCID,Nascimento Ana Paula Alves3ORCID,Farias Beatriz Oliveira3,Brito Andressa S Gonçalves3ORCID,Magaldi Mariana3,Gimenez Aline3ORCID,de Filippis Ivano34ORCID,Clementino Maysa Mandetta345ORCID,Bianco Kayo345ORCID,Saggioro Enrico26,Barrocas Paulo12

Affiliation:

1. National School of Public Health Sérgio Arouca, ENSP, Oswaldo Cruz Foundation—FIOCRUZ , Rio de Janeiro, RJ 4365 , Brazil

2. Post-Graduation Program in Public Health and Environment, Sergio Arouca National School of Public Health, Oswaldo Cruz Foundation , 1480 Leopoldo Bulhões Ave, Rio de Janeiro, RJ 21041-210 , Brazil

3. National Institute of Quality Control in Health—INCQS, Oswaldo Cruz Foundation—FIOCRUZ , Rio de Janeiro, RJ 4365 , Brazil

4. Fiocruz Genomic Network, Oswaldo Cruz Foundation—FIOCRUZ , Rio de Janeiro, RJ 4365 , Brazil

5. COVID-19 Monitoring Network in Wastewater , 09210-580, Brazil

6. Environmental Health Evaluation and Promotion Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation , 4365 Brasil Ave, Rio de Janeiro, RJ 21045-900 , Brazil

Abstract

Abstract Aims To investigate the occurrence of carbapenem-resistant Klebsiella pneumoniae (CRKP) and their clonal relationships from hospital and municipal wastewater treatment plants (WWTPs). Methods and results Eighteen Kl. pneumoniae strains recovered from three WWTPs were identified by matrix-assisted laser desorption-ionization–time of flight (MALDI-TOF). The antimicrobial susceptibility were evaluated by disk-diffusion and the carbapenemases production by Carbapenembac®. The carbapenemases genes were investigated by real-time PCR and the clonal relationship through multilocus sequence typing (MLST). Thirty nine % (7/18) of isolates were classified as multidrug-resistant (MDR), 61.1% (11/18) extensively drug-resistant (XDR), and 83.3% (15/18) showed carbapenemase activity. Three carbapenemase-encoding genes were found, blaKPC (55%), blaNDM (27.8%) and blaOXA-370 (11.1%) as well five sequencing types ST11, ST37, ST147, ST244, and ST281. ST11 and ST244, sharing four alleles were grouped into clonal complex 11 (CC11). Conclusions Our results show the importance of monitoring antimicrobial resistance in WWTPs effluents to minimize the risk of spreading bacterial load and ARGs in aquatic ecosystems, using advanced treatment technologies to reduce these emerging pollutants at WWTPs.

Funder

FAPERJ

CNPq

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

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