Clioquinol and 8-hydroxyquinoline-5-sulfonamide derivatives damage the cell wall of<i>Pythium insidiosum</i>-Reference-Cited by-同舟云学术

Clioquinol and 8-hydroxyquinoline-5-sulfonamide derivatives damage the cell wall ofPythium insidiosum

Published:2022-12-14 Issue:1 Volume:134 Page:
ISSN:1365-2672
Container-title:Journal of Applied Microbiology
language:en
Short-container-title:

Author:

Pippi Bruna¹,Zanette Régis A²,Joaquim Angélica R³,Krummenauer Maria E⁴,Merkel Simone²,Reginatto Paula³,Vainstein Marilene H⁴,Andrade Saulo F³⁵^ORCID,Fuentefria Alexandre M³⁵,Tondolo Juliana S M¹,Loreto Érico S¹,Santurio Janio M¹

Affiliation:

1. Graduation Program in Pharmacology, Federal University of Santa Maria , Santa Maria 97105-900, RS , Brazil

2. Graduation Program in Biological Sciences: Pharmacology and Therapeutics, Federal University of Rio Grande do Sul , Porto Alegre 90010-150, RS , Brazil

3. Graduation Program in Pharmaceutical Sciences, Federal University of Rio Grande do Sul , Porto Alegre, 90010-150, RS , Brazil

4. Biotechnology Center, Federal University of Rio Grande do Sul , Porto Alegre 90010-150, RS , Brazil

5. Graduation Program in Agricultural and Environmental Microbiology, Federal University of Rio Grande do Sul , Porto Alegre 90010-150, RS , Brazil

Abstract

AbstractAimsTo evaluate the antimicrobial activity and to determine the pharmacodynamic characteristics of three 8-hydroxyquinoline derivatives (8-HQs) against Pythium insidiosum, the causative agent of pythiosis.Methods and ResultsAntimicrobial activity was tested by broth microdilution and MTT assays. The antimicrobial mode of action was investigated using sorbitol protection assay, ergosterol binding assay, and scanning electron microscopy. Clioquinol, PH151, and PH153 were active against all isolates, with MIC values ranging from 0.25 to 2 µg ml-1. They also showed a time- and dose-dependent antimicrobial effect, damaging the P. insidiosum cell wall.ConclusionsTogether, these results reinforce the potential of 8-HQs for developing new drugs to treat pythiosis.

Funder

CNPq

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Link

https://academic.oup.com/jambio/article-pdf/134/1/lxac038/49094891/lxac038.pdf

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