Affiliation:
1. Division of Bioresources, Hokkaido University International Institute for Zoonosis Control , Sapporo 001-0020 , Japan
2. Department of Microbiology, Faculty of Public Health, Mahidol University , Bangkok 10400 , Thailand
3. International Collaboration Unit, Hokkaido University International Institute for Zoonosis Control , Sapporo 001-0020 , Japan
4. Division of Research Support, Hokkaido University Institute for Vaccine Research & Development , Sapporo 001-0020 , Japan
Abstract
Abstract
Aims
This research focused on assessing the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants and antimicrobial susceptibility in Salmonella strains isolated from Thai canal water.
Methods and results
From 2016 to 2020, 333 water samples were collected from six canals across Bangkok, Thailand. Salmonella spp. was isolated, PMQR genes were detected through polymerase chain reactions, and the antimicrobial susceptibility was examined using the disk diffusion method. The results indicated a 92.2% prevalence of Salmonella spp. in canal water, being serogroups B and C the most frequently detected. Overall, 35.3% of isolates harbored PMQR genes, being qnrS the most prevalent gene (97.2%, n = 137/141). Other PMQR genes, including qnrB, qnrD, oqxAB, and aac(6′)-Ib-cr, were detected. Notably, six isolates harbored multiple PMQR genes. Furthermore, 9.3% and 3.8% of the overall isolates were resistant to nalidixic acid (NAL) and ciprofloxacin (CIP), respectively. PMQR-positive isolates showed higher rates of non-susceptibility to both NAL (48.2%, n = 68/141) and CIP (92.2%, n = 130/141) compared to PMQR-negative isolates (NAL: 8.9%, n = 23/258; CIP: 11.2%, n = 30/258).
Conclusions
The high prevalence of Salmonella spp., significant PMQR-positive, and reduced susceptibility isolates in canal water is of public health concern in Bangkok.
Funder
Japan Agency for Medical Research and Development
Japan Society for Promotion of Science
Publisher
Oxford University Press (OUP)