Isolation, characterization of Enterococcus phages and their application in control of E. faecalis in milk

Author:

Wang Chen1,Zhao Junxin12,Lin Yunzhi1,Yuan Lu1,El-Telbany Mohamed1,Maung Aye Thida1,Abdelaziz Marwa Nabil Sayed1ORCID,Masuda Yoshimitsu3,Honjoh Ken-ichi3,Miyamoto Takahisa3

Affiliation:

1. Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University , Fukuoka 819-0395 , Japan

2. State Key Laboratory of Food Science and Resources, Nanchang University , Nanchang 330047 , China

3. Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 819-0395 , Japan

Abstract

Abstract Aims Isolation and characterization of Enterococcus phages and application of phage cocktail to control E. faecalis in milk. Methods and Results For phage isolations, double layer agar method was used. Host range of the phages were determined by the spot test. Twelve phages with varying host ranges were isolated. Phages PEF1, PEF7b, and PEF9 with different host ranges and lytic activities were selected for phage cocktails. Compared to two-phages cocktails tested, the cocktail containing all the three phages displayed stronger antibacterial and biofilm removal activities. The cocktail treatment reduced viable E. faecalis in biofilm by 6 log within 6 h at both 30°C and 4°C. In milk, the cocktail gradually reduced the viable count of E. faecalis and the count reached below the lower limit of detection at 48 h at 4°C. Conclusion The strong bactericidal and biofilm removal activities of the phage cocktail suggest the potential of this cocktail as a natural biocontrol agent for combating E. faecalis in milk.

Funder

Japan Science and Technology Agency

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

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