The occurrence and genomic characteristics of the blaIMI-1 carbapenemase-producing Enterobacter cloacae complex retrieved from natural water sources in central Thailand

Author:

Songsaeng Wipawee1ORCID,Prapasarakul Nuvee23,Wongsurawat Thidathip4,Sirichokchatchawan Wandee13ORCID

Affiliation:

1. College of Public Health Sciences (CPHS), Chulalongkorn University , Bangkok 10330 , Thailand

2. Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University , Bangkok 10330 , Thailand

3. Center of Excellence in Diagnosis and Monitoring of Animal Pathogens (DMAP) , Bangkok 10330 , Thailand

4. Faculty of Medicine, Siriraj Hospital, Mahidol University , Bangkok 10700 , Thailand

Abstract

Abstract Aim Carbapenem resistance among Enterobacteriaceae is a serious threat to humans worldwide. This study aims to evaluate the phenotypic and genotypic characterization of carbapenemase-producing Enterobacter cloacae complex (ECC) retrieved from water sources in the central part of Thailand. Methods and results Samples were collected from water bodies surrounding farms and communities in central Thailand. The species were identified by using MALDI-TOF MS. The minimum inhibitory concentration (MIC) and antibiotic susceptibility were determined. The carbapenemase-producing genes were detected by PCR and whole genome sequencing (WGS). ECC with chromosome-encoded blaIMI-1 carbapenemase were detected. These isolates were resistant to last-resort antibiotics such as carbapenems and colistin as well as penicillin. In addition, all blaIMI-1 genes isolated from this study were found to be associated with chromosomally integrated Xer-dependent integrative mobile elements (IMEXs). Conclusion These findings highlight the diversity and dissemination of carbapenemases-producing Enterobacterales in environmental sources. With the increasing detection of carbapenemase genes worldwide, we should be aware of the blaIMI-producing E. cloacae complex with a high resistance profile and the ability to mobilize within the environment.

Funder

The 100th Anniversary Chulalongkorn University Fund

The 90th Anniversary of Chulalongkorn University Fund

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

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