Encapsulation of Pseudomonas aeruginosa strain KBN12 decolourizes and bioremediates brilliant blue dye mediated toxicity in mung bean (Vigna radiata L.)

Abstract

Abstract Aim The aim of this study was to explore the decolourization and bioremediation ability of non-encapsulated and encapsulated Pseudomonas aeruginosa (strain KBN 12) against the azo dye brilliant blue (BB). Methods and results Six efficient BB dye-decolourizing bacteria were isolated from textile dye effluent. The most efficient free cells of P. aeruginosa KBN 12 along with the optimized conditions such as carbon source (maltose: 5 g L−1), and nitrogen source (ammonium chloride: 4 g L−1) at pH 6 at 37°C decolourized 72.69% of BB dye aerobically after 9 days of incubation under static conditions. Encapsulated (calcium alginate) P. aeruginosa KBN 12 decolourized 87.67% of BB dye aerobically after 9 days of incubation under the same optimized conditions. Fourier-transform infrared spectroscopy (FTIR) and gas chromatography (GC) analysis of the chemical structure of BB dye after decolourization found changes in functional and chemical groups. Phytotoxicity and soil respiration enzyme assays revealed that the decolourized dye or dye products were less toxic than the pure BB dye. Conclusion The encapsulation of P. aeruginosa KBN 12 proved to be an effective method for BB dye decolourization or remediation.