Affiliation:
1. Universidade Federal de Pernambuco-UFPE, Laboratório de Microbiologia, Área de Medicina Tropical, Centro de Ciências Médicas—CCM , 50670-901 , Recife-PE, Brasil
2. Secretaria de Saúde do Estado de Pernambuco , 50751-230, Recife-PE , Brazil
Abstract
Abstract
Aims
Investigated and compared the occurrence of virulence genes fimH, mrkD, irp2, entB, cps, rmpA, and wabG, resistance genes blaKPC and blaNDM, and the genetic variability and clonal relationship of 29 Klebsiella pneumoniae clinical isolates of patients with and without COVID-19, from a hospital in Brazil.
Methods and results
All isolates were resistant to beta-lactams. The genes were investigated by PCR, and for molecular typing, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and MLST were used. The detection of blaNDM was greater (n = 23) when compared to that of blaKPC (n = 14). The virulence genes that most occurred were fimH, entB, cps, and wabG, which are responsible for adhesins, siderophore enterobactin, capsule, and lipopolysaccharides, respectively. Among the isolates, 21 distinct genetic profiles were found by ERIC-PCR, with multiclonal dissemination. Four isolates belonged to the ST11 clone.
Conclusions
The occurrence of the ST11 is worrying as it is a high-risk clone involved in the dissemination of virulent strains throughout the world.
Publisher
Oxford University Press (OUP)
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