The Composition of the Fecal and Mucosa-adherent Microbiota Varies Based on Age and Disease Activity in Ulcerative Colitis

Author:

Malham Mikkel12ORCID,Vestergaard Marie V34,Bataillon Thomas3ORCID,Villesen Palle35,Dempfle Astrid6ORCID,Bang Corinna7ORCID,Engsbro Anne Line8,Jakobsen Christian12,Franke Andre7,Wewer Vibeke12,Thingholm Louise B7,Petersen Andreas M18910

Affiliation:

1. Department of Pediatric and Adolescent Medicine, Copenhagen University Hospital–Amager and Hvidovre , Hvidovre , Denmark

2. Copenhagen Center for Inflammatory Bowel Disease in Children, Adolescents, and Adults, Copenhagen University Hospital–Amager and Hvidovre , Hvidovre , Denmark

3. Bioinformatics Research Centre, Aarhus University , 8000 Aarhus C , Denmark

4. Center for Molecular Prediction of Inflammatory Bowel Disease, PREDICT, Department of Clinical Medicine, Aalborg University , Copenhagen , Denmark

5. Department of Clinical Medicine, Aarhus University Hospital , 8000 Aarhus C , Denmark

6. Institut für Medizinische Informatik und Statistik, Universitätsklinikum Schleswig-Holstein , 24105 Kiel , Germany

7. Institute of Clinical Molecular Biology, Christian-Albrechts-University of Kiel , 24105 Kiel , Germany

8. Department of Clinical Microbiology, Copenhagen University Hospital–Hvidovre , Denmark

9. Gastrounit, Medical Division, Copenhagen University Hospital–Hvidovre , Denmark

10. Department of Clinical Medicine, University of Copenhagen , Copenhagen , Denmark

Abstract

Abstract Background Pediatric-onset ulcerative colitis (pUC) represents a more aggressive disease phenotype compared with adult-onset UC. We hypothesized that this difference can, in part, be explained by the composition of the microbiota. Methods In a prospective, longitudinal study, we included pediatric (N = 30) and adult (N = 30) patients with newly or previously (>1 year) diagnosed UC. We analyzed the microbiota composition in the mucosa-adherent microbiota at baseline, using 16S rRNA gene sequencing, and the fecal microbiota at baseline and at 3-month intervals, using shotgun metagenomics. Results For fecal samples, the bacterial composition differed between pUC and aUC in newly diagnosed patients (β-diversity, Bray Curtis: R2 = 0.08, P = .02). In colon biopsies, microbial diversity was higher in aUC compared with pUC (α-diversity, Shannon: estimated difference 0.54, P = .006). In the mucosa-adherent microbiota, Alistipes finegoldii was negatively associated with disease activity in pUC while being positively associated in aUC (estimate: −0.255 and 0.098, P = .003 and P = .02 in pUC and aUC, respectively). Finally, we showed reduced stability of the fecal microbiota in pediatric patients, evidenced by a different composition of the fecal microbiota in newly and previously diagnosed pUC, a pattern not found in adults. Conclusions Our results indicate that pediatric UC patients have a more unstable fecal microbiota and a lower α diversity than adult patients and that the microbiota composition differs between aUC and pUC patients. These findings offer some explanation for the observed differences between pUC and aUC and indicate that individualized approaches are needed if microbiota modifications are to be used in the future treatment of UC.

Funder

Aage and Johann Louis-Hansens Foundation

Aase and Ejnar Danielsens Foundation

German Research Foundation

Publisher

Oxford University Press (OUP)

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3