Affiliation:
1. Mendel University of Agriculture and Forestry, Department of Chemistry and Biochemistry, Zemědělská 1, CZ-613 00 Brno, Czech Republic
Abstract
Abstract
A fast, selective, and sensitive liquid chromatographic (LC) method was developed for determination of derivatives of benzoic and cinnamic acids (gallic, protocatechuic, 2,3,4–trihydroxybenzoic, 4-hydroxybenzoic, vanillic, caffeic, syringic, 4-coumaric, ferulic, sinapic, benzoic, 2-coumaric, cinnamic acids, and 4-hydroxybenzaldehyde and vanillin) in soil samples. The method for sample pretreatment is based on temperature-controlled extraction with water (pH 5.6) for 60 min. Extracts were preconcentrated and purified by solid-phase extraction on OASIS HLB sorbent, with subsequent separation and quantification of individual substances by LC with UV diode-array detection. Limits of detection (3 signal-to-noise LODs) better than 65 ng/g (dry weight) and recoveries from 88 to 99% were found for each compound at absorbance 280 nm. The method was used for determination of bioavailable phenolic compounds in different soil samples.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
3 articles.
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