A multiplex PCR assay for the identification of 5 sibling species of the Lindesayi Complex of the genus <i>Anopheles</i> (Diptera: Culicidae) in Bhutan-Reference-Cited by-同舟云学术

A multiplex PCR assay for the identification of 5 sibling species of the Lindesayi Complex of the genus Anopheles (Diptera: Culicidae) in Bhutan

Published:2023-07-07 Issue:5 Volume:60 Page:924-930
ISSN:0022-2585
Container-title:Journal of Medical Entomology
language:en
Short-container-title:

Author:

Zhou Li¹,Namgay Rinsin²^ORCID,Saeung Atiporn¹,Saingamsook Jassada¹,Phanitchakun Thanari¹,Harbach Ralph E³,Somboon Pradya¹^ORCID

Affiliation:

1. Center of Insect Vector Study, Department of Parasitology, Faculty of Medicine, Chiang Mai University , Chiang Mai 50200 , Thailand

2. Vector-borne Disease Control Programme, Ministry of Health , Gelephu 31101 , Bhutan

3. Department of Science, Natural History Museum , Cromwell Road, London SW7 5BD , UK

Abstract

Abstract Five sibling species of the Lindesayi Complex of the genus Anopheles have been discovered in Bhutan: An. druki Somboon, Namgay & Harbach, An. himalayensis Somboon, Namgay & Harbach, An. lindesayi Giles, An. lindesayi species B, and An. thimphuensis Somboon, Namgay & Harbach. The species are morphologically similar in the adult and/or immature stages. This study aimed to develop a multiplex PCR assay to identify the 5 species. Allele-specific primers were designed for specific nucleotide segments of ITS2 sequences previously reported for each species. The assay provided products of 183 bp for An. druki, 338 bp for An. himalayensis, 126 bp for An. lindesayi, 290 bp for An. lindesayi species B, and 370 bp for An. thimphuensis. The use of the assay produced consistent results. The assay is relatively inexpensive, enables the rapid identification of a large number of specimens, and will foster further studies of the Lindesayi Complex.

Funder

Faculty of Medicine, Chiang Mai University

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Insect Science,General Veterinary,Parasitology

Link

https://academic.oup.com/jme/article-pdf/60/5/924/51471682/tjad089.pdf

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