Analysis of Myo-inositol in Dry Beans by Gas Chromatography of Its Hexaacetate

Abstract

Abstract A method has been developed for the analysis of myo-inositol in California small white beans, pinto beans, and 2 pinto bean flake products by gas-liquid chromatography of myo-inositol hexaacetate. The identity of myoinositol hexaacetate from bean samples was confirmed by mass spectrometry and chromatographic retention time. A standard curve prepared by plotting peak height vs. μg myoinositol hexaacetate was linear over the range studied, 0.2 to 0.6 μg. Peak heights were measurable in all samples, although inositol hexaacetate was not completely separated in California small white bean samples. An internal standard, perseitol, was used to correct for losses in the procedure. Hydrolysis of phytin was negligible during sample preparation or analysis. Recoveries of 12 to 40 μg inositol from 40 mg samples of California small white beans averaged 113%. These beans contained an average of 71 ppm inositol compared to 215 ppm inositol in similarly prepared pinto beans.