Determination of Avermectin Bi in Biological Samples by Immunoaffinity Column Cleanup and Liquid Chromatography with UV Detection

Abstract

Abstract A simple and specific procedure for determination of avermectin B1 in biological samples is described. A specific anti-avermectin B1 immunosorbent was developed. Avermectin B1 was extracted from cattle plasma, cattle meat, and pear samples. The extracts were cleaned up on a solid-phase extraction column (C18) and an immunoaffinity column. Avermectin B1 was determined by reversedphase liquid chromatography with UV detection at 245 nm. Recoveries of avermectin Bi from samples spiked at levels of 6 and 60 parts per billion (ppb) ranged from 80 to 86%, with coefficients of variation of 5-14%. The limit of detection was 2 ppb. The procedure provides a simple and sensitive method for monitoring avermectin B1 residues in biological matrixes.