Transcription elongator SPT6L regulates the occupancies of the SWI2/SNF2 chromatin remodelers SYD/BRM and nucleosomes at transcription start sites in Arabidopsis

Author:

Shu Jie1ORCID,Ding Ning12,Liu Jun3ORCID,Cui Yuhai45,Chen Chen12

Affiliation:

1. Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences , Guangzhou, Guangdong 510650, China

2. University of the Chinese Academy of Sciences , Beijing 100049, China

3. Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences , Guangzhou, Guangdong 510640, China

4. Agriculture and Agri-Food Canada, London Research and Development Centre, London , Ontario N5V 4T3, Canada

5. Department of Biology, Western University , London, Ontario N6A 5B7, Canada

Abstract

Abstract Chromatin remodelers have been thought to be crucial in creating an accessible chromatin environment before transcription activation. However, it is still unclear how chromatin remodelers recognize and bind to the active regions. In this study, we found that chromatin remodelers SPLAYED (SYD) and BRAHMA (BRM) interact and co-occupy with Suppressor of Ty6-like (SPT6L), a core subunit of the transcription machinery, at thousands of the transcription start sites (TSS). The association of SYD and BRM to chromatin is dramatically reduced in spt6l and can be restored mainly by SPT6LΔtSH2, which binds to TSS in a RNA polymerase II (Pol II)-independent manner. Furthermore, SPT6L and SYD/BRM are involved in regulating the nucleosome and Pol II occupancy around TSS. The presence of SPT6L is sufficient to restore the association of the chromatin remodeler SYD to chromatin and maintain normal nucleosome occupancy. Our findings suggest that the two chromatin remodelers can form protein complexes with the core subunit of the transcription machinery and regulate nucleosome occupancy in the early transcription stage.

Funder

National Natural Science Foundation of China

Natural Science and Engineering Research Council of Canada

Agriculture and Agri-Food Canada

Publisher

Oxford University Press (OUP)

Subject

Genetics

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