Fluorometric Determination of Estradiol Valerate in Sesame Oil or Ethyl Oleate Injectables. II. Collaborative Study

Abstract

Abstract A fluorometric method for determining estradiol valerate in sesame oil or ethyl oleate was subjected to collaborative study. The method utilizes a nitromethane-Celite column to isolate and purify the steroid, which is then measured fluorometrically. Four commercial samples and 3 synthetic samples, varying in concentration from 4 to 20 mg/ml, were used in the study. The collaborative results were acceptable: Coefficients of variation ranged from 2.48 to 3.55%; average recoveries for the synthetics ranged from 98.6 to 102%. The method has been adopted as official first action.