Liquid Chromatographic Determination of Benzo[a]pyrene in Total Particulate Matter of Cigarette Smoke

Abstract

Abstract The benzo[a]pyrene (BaP) delivery of reference and commercially 1 available tobacco cigarettes, as well as reference and placebo marijuana cigarettes, is determined using a sequential liquid chromatographic liquid chromatographic procedure. The total particulate matter of sample cigarette smoke is collected using a Cambridge filter pad, which isultrasonically extracted with acetone. The resulting extract is filtered, then fractionated using semipreparative-scale normal phase liquid chromatography (LC). Quantitative determination is achieved using analytical-scale reverse phase LC equipped with a fluorescence detector. The method is precise (± 10-15% relative standard deviation) and yields 85% or better BaP recovery at the μg/cig. level. A single , pad may be analyzed in 8 person-hours, while a more typical lot of 12 pads (6 pads each for 2 cigarette brands) may be analyzed in 10 person days.