Pushing the limits of de novo genome assembly for complex prokaryotic genomes harboring very long, near identical repeats

Author:

Schmid Michael12,Frei Daniel1,Patrignani Andrea3,Schlapbach Ralph3,Frey Jürg E1,Remus-Emsermann Mitja N P45,Ahrens Christian H12ORCID

Affiliation:

1. Agroscope, Molecular Diagnostics, Genomics & Bioinformatics, Wädenswil CH-8820, Switzerland

2. SIB Swiss Institute of Bioinformatics, Wädenswil CH-8820, Switzerland

3. Functional Genomics Center Zurich, University of Zurich & ETH Zurich, Zurich CH-8057, Switzerland

4. School of Biological Sciences, University of Canterbury, Christchurch 8140, New Zealand

5. Biomolecular Interaction Centre, University of Canterbury, Christchurch, 8140, New Zealand

Funder

Swiss National Science Foundation

Publisher

Oxford University Press (OUP)

Subject

Genetics

Reference61 articles.

1. Coming of age: ten years of next-generation sequencing technologies;Goodwin;Nat. Rev. Genet.,2016

2. The Genomes OnLine Database (GOLD) v.5: a metadata management system based on a four level (meta)genome project classification;Reddy;Nucleic Acids Res.,2015

3. A complete bacterial genome assembled de novo using only nanopore sequencing data;Loman;Nat. Methods,2015

4. Completing bacterial genome assemblies with multiplex MinION sequencing;Wick;Microb. Genom.,2017

5. Optimal DNA shotgun sequencing: Noisy reads are as good as noiseless reads;Motahari,2013

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