Adaptation of a eukaryote-like ProRS to a prokaryote-like tRNAPro

Author:

Ivanesthi Indira Rizqita1,Latifah Emi1,Amrullah Luqman Fikri1,Tseng Yi-Kuan2,Chuang Tsung-Hsien3ORCID,Pan Hung-Chuan4,Yang Chih-Shiang1,Liu Shih-Yang1,Wang Chien-Chia1ORCID

Affiliation:

1. Department of Life Sciences, National Central University , Zhongli District, Taoyuan 320317, Taiwan

2. Graduate Institute of Statistics, National Central University , Zhongli District, Taoyuan 320317, Taiwan

3. Immunology Research Center, National Health Research Institutes , Zhunan Town, Miaoli 35053, Taiwan

4. Department of Neurosurgery, Taichung Veterans General Hospital , Taichung 407219, Taiwan

Abstract

Abstract Prolyl-tRNA synthetases (ProRSs) are unique among aminoacyl-tRNA synthetases (aaRSs) in having two distinct structural architectures across different organisms: prokaryote-like (P-type) and eukaryote/archaeon-like (E-type). Interestingly, Bacillus thuringiensis harbors both types, with P-type (BtProRS1) and E-type ProRS (BtProRS2) coexisting. Despite their differences, both enzymes are constitutively expressed and functional in vivo. Similar to BtProRS1, BtProRS2 selectively charges the P-type tRNAPro and displays higher halofuginone tolerance than canonical E-type ProRS. However, these two isozymes recognize the primary identity elements of the P-type tRNAPro―G72 and A73 in the acceptor stem―through distinct mechanisms. Moreover, BtProRS2 exhibits significantly higher tolerance to stresses (such as heat, hydrogen peroxide, and dithiothreitol) than BtProRS1 does. This study underscores how an E-type ProRS adapts to a P-type tRNAPro and how it may contribute to the bacterium's survival under stress conditions.

Funder

National Science and Technology Council

Taipei Veterans General Hospital

Publisher

Oxford University Press (OUP)

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3