MUT-7 exoribonuclease activity and localization are mediated by an ancient domain

Author:

Busetto Virginia12ORCID,Pshanichnaya Lizaveta34ORCID,Lichtenberger Raffael12,Hann Stephan5ORCID,Ketting René F36ORCID,Falk Sebastian12ORCID

Affiliation:

1. Max Perutz Labs, Vienna Biocenter Campus (VBC) , Dr.-Bohr-Gasse 9, 1030  Vienna , Austria

2. Department of Structural and Computational Biology, Max Perutz Labs, University of Vienna, Campus Vienna Biocenter 5 , 1030  Vienna , Austria

3. Biology of Non-coding RNA Group, Institute of Molecular Biology , Ackermannweg 4, 55128  Mainz , Germany

4. International PhD Programme on Gene Regulation, Epigenetics & Genome Stability , Mainz , Germany

5. Institute of Analytical Chemistry, Department of Chemistry, University of Natural Resources and Life Sciences, Vienna , Muthgasse 18, 1190 Vienna , Austria

6. Institute of Developmental Biology and Neurobiology, Johannes Gutenberg University , 55099 Mainz , Germany

Abstract

Abstract The MUT-7 family of 3′–5′ exoribonucleases is evolutionarily conserved across the animal kingdom and plays essential roles in small RNA production in the germline. Most MUT-7 homologues carry a C-terminal domain of unknown function named MUT7-C appended to the exoribonuclease domain. Our analysis shows that the MUT7-C is evolutionary ancient, as a minimal version of the domain exists as an individual protein in prokaryotes. In animals, MUT7-C has acquired an insertion that diverged during evolution, expanding its functions. Caenorhabditis elegans MUT-7 contains a specific insertion within MUT7-C, which allows binding to MUT-8 and, consequently, MUT-7 recruitment to germ granules. In addition, in C. elegans and human MUT-7, the MUT7-C domain contributes to RNA binding and is thereby crucial for ribonuclease activity. This RNA-binding function most likely represents the ancestral function of the MUT7-C domain. Overall, this study sheds light on MUT7-C and assigns two functions to this previously uncharacterized domain.

Funder

Austrian Science Fund

European Union’s Framework Programme for Research and Innovation Horizon 2020

Marie Curie Skłodowska

Deutsche Forschungsgemeinschaft

University of Vienna

Publisher

Oxford University Press (OUP)

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