A fluorescent complex with peptide nucleic acids modified by boronic acid and its ligand detects target RNA in cells-Reference-Cited by-同舟云学术

A fluorescent complex with peptide nucleic acids modified by boronic acid and its ligand detects target RNA in cells

Published:2024-08-23 Issue:9 Volume:53 Page:
ISSN:0366-7022
Container-title:Chemistry Letters
language:en
Short-container-title:

Author:

Ishii Koki¹,Hattori Yoshihide²,Shigeto Hajime³,Yamamura Shohei³,Kitamatsu Mizuki¹

Affiliation:

1. Department of Applied Chemistry, Kindai University , 3-4-1 Kowakae, Higashi-Osaka, Osaka 577-8502 , Japan

2. Research Center of Boron Neutron Capture Therapy, Osaka Metropolitan University , 1-1 Gakuen-cho, Nakaku, Sakai, Osaka 599-8231 , Japan

3. Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST) , 2217-14 Hayashi-cho, Takamatsu, Kagawa 761-0395 , Japan

Abstract

Abstract We have developed peptide nucleic acids (PNAs) modified with boronic acid (Boa) and its ligand 2-(pyridin-2-yl)phenol (Pyp) as a probe for fluorescence detection of a target nucleic acid. Boa and Pyp successfully showed fluorescence by complexing via hybridization with PNA and the target. This fluorescent PNA probe also successfully responded to the target RNA in cells.

Funder

2023 Kindai University Research Enhancement

Publisher

Oxford University Press (OUP)

Link

https://academic.oup.com/chemlett/advance-article-pdf/doi/10.1093/chemle/upae169/58906451/upae169.pdf

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