Microstructural Analysis of Collagenous Structures in Relapsed Clubfoot Tissue

Author:

Vondrášek David12ORCID,Hadraba Daniel123ORCID,Přibyl Jan4ORCID,Eckhardt Adam2,Ošťádal Martin5,Lopot František13,Jelen Karel1,Doubková Martina26ORCID,Knitlová Jarmila2ORCID,Novotný Tomáš78ORCID,Janáček Jiří2ORCID

Affiliation:

1. Faculty of Physical Education and Sport, Charles University , Prague 16252 , Czech Republic

2. Institute of Physiology of the Czech Academy of Sciences , Prague 4 14220 , Czech Republic

3. Faculty of Mechanical Engineering, Czech Technical University in Prague , Prague 16607 , Czech Republic

4. Core Facility NanoBiotechnology, CEITEC MU, Masaryk University , Brno 62500 , Czech Republic

5. Department of Orthopaedics, Hospital Bulovka, Charles University , Prague 18081 , Czech Republic

6. Second Faculty of Medicine, Charles University , Prague 15006 , Czech Republic

7. Department of Orthopaedics, University J.E. Purkinje and Masaryk Hospital , Usti nad Labem 40113 , Czech Republic

8. Department of Histology and Embryology, Second Faculty of Medicine, Charles University , Prague 15006 , Czech Republic

Abstract

Abstract Talipes equinovarus congenitus (clubfoot) is frequently defined as a stiff, contracted deformity, but few studies have described the tissue from the point of view of the extracellular matrix, and none have quantified its mechanical properties. Several researchers have observed that clubfoot exhibits signs of fibrosis in the medial side of the deformity that are absent in the lateral side. Our study aims to quantify the differences between the medial and lateral side tissue obtained from relapsed clubfoot during surgery in terms of the morphological and mechanical properties of the tissue. Combining methods of optical and atomic force microscopy, our study revealed that the medial side has a higher Young’s modulus, contains more collagen and less adipose tissue and that the collagen fibers propagate at a higher frequency of the crimp pattern after surgical dissection of the tissue. Our study offers a multi-correlative approach that thoroughly investigates the relapsed clubfoot tissue.

Publisher

Oxford University Press (OUP)

Subject

Instrumentation

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