Measuring the Effect of Ice Thickness and Microscope Configuration on Resolution in Single Particle Cryo-EM

Author:

Chua Eugene Y D1,Neselu Kasahun1,Wang Bing2,Rice William J23,Potter Clinton S4,Carragher Bridget4

Affiliation:

1. Simons Electron Microscopy Center, New York Structural Biology Center , New York, NY , USA

2. Cryo-Electron Microscopy Core, New York University Grossman School of Medicine , New York, NY USA

3. Department of Cell Biology, New York University Grossman School of Medicine , New York, NY , USA

4. Chan Zuckerberg Imaging Institute , Redwood City, CA , USA

Publisher

Oxford University Press (OUP)

Subject

Instrumentation

Reference5 articles.

1. Routine single particle CryoEM sample and grid characterization by tomography

2. Thicker Ice Improves the Integrity and Angular Distribution of CDC48A Hexamers on Cryo-EM Grids;Huntington;Frontiers in Molecular Biosciences,2022

3. Single-particle cryo-EM data acquisition by using direct electron detection camera;Wu;Microscopy,2016

4. IceBreaker: Software for high-resolution singleparticle cryo-EM with non-uniform ice;Olek;Structure,2022

5. Measuring the effect of ice thickness on resolution in single particle cryo-EM;Neselu;Journal of Stuctural Biology: X,2023

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