Detection, Identification, and Quantitation of Organic Compounds with Bioanalytical Systems Based on Mass Spectrometry

Abstract

Abstract Bioanalytical systems which include a gas chromatograph, a mass spectrometer, and a computer provide the most effective analytical methods for organic compounds other than macromolecules. The use of computer and microprocessor/computer instrumental combinations is increasing. Electron impact mass spectrometry is used for identification purposes; chemical ionization is best for quantitative work. Current studies indicate that negative ion mass spectrometry with non-filament sources will be useful in analyses for toxic agents (pesticides, fungicides, herbicides). Future analytical systems will have both positive and negative ion mass analyzer capabilities. Sample preparation methods depend on the type of analysis. Liquid chromatography is useful when the concentration is low. Open tubular glass capillary columns are coming into wider use, both in gas chromatography alone and in combined systems. The limits of detection are now in the subpicogram range, but the determining factor is usually the nature of the compounds under analysis and the extent of adsorption.