Affiliation:
1. From the Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , Shijingshan District, Beijing , People's Republic of China
Abstract
Abstract
Background
Some adipocytes undergo dedifferentiation after fat transplantation, and this may affect the survival of fat grafts. However, this effect has not been adequately studied.
Objectives
This study aimed to clarify the effect of promoting the dedifferentiation of mature adipocytes on the survival of fat grafts.
Methods
Mature adipocytes and adipose stem cells (ASCs) were treated with OSI-906 (a specific inhibitor of insulin receptor and insulin-like growth factor-1 receptor) in vitro, and then the dedifferentiation of mature adipocytes and the proliferation of ASCs were evaluated. In the in vivo experiment, human lipoaspirates mixed with phosphate-buffered saline (Group A) or OSI-906 (Group B) were compared in nude mice. Grafts were harvested at 2, 8, and 12 weeks, and volume retention rate, histologic, and immunohistochemical analyses were conducted.
Results
OSI-906 can promote the dedifferentiation of mature adipocytes and inhibit the proliferation of ASCs. At 12 weeks, Group B showed a better volume retention rate (mean [standard deviation, SD], 62.3% [7.61%]) than group A (47.75% [6.11%]) (P < .05). Moreover, viable adipocytes and vascularization showed greater improvement in Group B than in Group A.
Conclusions
This study suggests that promoting the dedifferentiation of mature adipocytes can improve the survival rate and quality of fat grafts.
Publisher
Oxford University Press (OUP)
Cited by
3 articles.
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