A Confirmed Case of SARS-CoV-2 Pneumonia With Negative Routine Reverse Transcriptase–Polymerase Chain Reaction and Virus Variation in Guangzhou, China

Author:

Li Zhengtu1,Li Yinhu2,Chen Lingdan1,Li Shaoqiang1,Yu Le2,Zhu Airu1,Yang Feng1,Jiang Qian1,Chen Liyan1,Zhao Jincun1,Lu Wenju1,Zhong Nanshan1,Ye Feng1ORCID

Affiliation:

1. State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

2. Beijing YuanShengKangTai (ProtoDNA) Genetech Co Ltd, Beijing, China

Abstract

Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine reverse transcriptase -polymerase chain reaction (RT-PCR) detection of SARS-CoV-2. Methods However, we report a confirmed case of SARS-CoV-2 pneumonia with a negative routine RT-PCR. Results This case was finally diagnosed by nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variations of SARS-CoV-2 were found. Conclusions This case highlighted that false-negative results could be present in routine RT-PCR diagnosis, especially with virus variation. Currently, nanopore pathogen sequencing and antibody detection have been found to be effective in clinical diagnosis.

Funder

Department of Science and Technology of Guangdong Province

Traditional Chinese Medicine Bureau of Guangdong Province

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Microbiology (medical)

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