Mob4 is essential for spermatogenesis in Drosophila melanogaster

Author:

Santos Inês B123,Wainman Alan4,Garrido-Maraver Juan1235,Pires Vanessa123,Riparbelli Maria Giovanna6,Kovács Levente7,Callaini Giuliano6,Glover David M7,Tavares Álvaro A123

Affiliation:

1. Faculty of Medicine and Biomedical Sciences, University of Algarve , 8005-139 Faro , Portugal

2. Centre for Biomedical Research (CBMR), University of Algarve , 8005-139 Faro , Portugal

3. Algarve Biomedical Center (ABC), University of Algarve , 8005-139 Faro , Portugal

4. Sir William Dunn School of Pathology, University of Oxford , South Parks Road, Oxford, OX1 3RE , UK

5. Centro Andaluz de Biología del Desarrollo, Universidad Pablo de Olavide , 41013 Sevilla , Spain

6. University of Siena, Department of Life Sciences , Via Aldo Moro, 2, 53100 Siena , Italy

7. Division of Biology and Biological Engineering, California Institute of Technology , 91125 Pasadena, California

Abstract

Abstract Gamete formation is essential for sexual reproduction in metazoans. Meiosis in males gives rise to spermatids that must differentiate and individualize into mature sperm. In Drosophila melanogaster, individualization of interconnected spermatids requires the formation of individualization complexes that synchronously move along the sperm bundles. Here, we show that Mob4, a member of the Mps-one binder family, is essential for male fertility but has no detectable role in female fertility. We show that Mob4 is required for proper axonemal structure and its loss leads to male sterility associated with defective spermatid individualization and absence of mature sperm in the seminal vesicles. Transmission electron micrographs of developing spermatids following mob4RNAi revealed expansion of the outer axonemal microtubules such that the 9 doublets no longer remained linked to each other and defective mitochondrial organization. Mob4 is a STRIPAK component, and male fertility is similarly impaired upon depletion of the STRIPAK components, Strip and Cka. Expression of the human Mob4 gene rescues all phenotypes of Drosophila mob4 downregulation, indicating that the gene is evolutionarily and functionally conserved. Together, this suggests that Mob4 contributes to the regulation of the microtubule- and actin-cytoskeleton during spermatogenesis through the conserved STRIPAK complex. Our study advances the understanding of male infertility by uncovering the requirement for Mob4 in sperm individualization.

Funder

Algarve 2020 Program

FEDER Funds

Operational Program for Competitiveness Factors—COMPETE 2020

National Funds

FCT

Foundation for Science and Technology

FCT fellowship

Cancer Research UK

Ministero dell’Istruzione

dell’Università e della Ricerca

Wellcome

NIH

Publisher

Oxford University Press (OUP)

Subject

Genetics

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