Affiliation:
1. Institut de Génétique et de Biologie Microbiennes, Université de Lausanne, CH-1005 Lausanne, Switzerland
Abstract
Abstract
Sequencing of the complete Bacillus subtilis chromosome revealed the presence of ∼4100 genes, 1000 of which were previously identified and mapped by classical genetic crosses. Comparison of these experimentally determined positions to those derived from the nucleotide sequence showed discrepancies reaching up to 24° (∼280 kb). The size of these discrepancies as a function of their position along the chromosome is not random but, apparently, reveals some periodicity. Our analyses demonstrate that the discrepancies can be accounted for by inaccurate positioning of the early reference markers with respect to which all subsequently identified loci were mapped by transduction and transformation. We conclude (i) that specific DNA sequences, such as recombination hotspots or presence of heterologous DNA, had no detectable effect on the results obtained by classical mapping, and (ii) that PBS1 transduction appears to be an accurate and unbiased mapping method in B. subtilis.
Publisher
Oxford University Press (OUP)
Cited by
2 articles.
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