SINGLE-TRAIT AND ANTAGONISTIC INDEX SELECTION FOR LITTER SIZE AND BODY WEIGHT IN MICE

Abstract

ABSTRACT Individual selection based on female performance only was conducted in four lines of mice: L+ for increased litter size, W+ for increased 6-week body weight, L-W+ for a selection index aimed at decreasing litter size and increasing 6-week body weight and L+W- for a selection index aimed at increasing litter size and decreasing 6-week body weight. A fifth line (K) served as an unselected control. All litters were standardized to eight mice at one day of age. Expected heritability was based on twice the regression of offspring on dam (h  2  d), which contains additive genetic variance due to direct (α2Ao) and maternal (α2  Am) effects and their covariance (αAoAm). Responses and correlated responses were measured either deviated (method 1) or not deviated (method 2) from the control line. Realized heritabilities (h2  R) for litter size were 0.19 ± 0.04 (1) and 0.16 ± 0.03 (2), which were similar to h2  d of 0.17 ± 0.04. The h2  R for 6-week body weight of 0.55 ± 0.07 (1) and 0.44 ± 0.07 (2) agreed with h2  d of 0.42 ± 0.02. Realized genetic correlations (r*GR) between litter size and 6-week body weight calculated from the double-selection experiment were 0.52 ± 0.10 (1) and 0.52 ± 0.13 (2), which were not significantly different from the base population estimate of r*Gd = 0.63 ± 0.14. Divergence (L-W+ minus L+W-) in the antagonistic index selection lines was 0.21 ± 0.01 index units (I = 0.305 PW - 0.436 PL, where PW and PL are the phenotypic values for 6-week body weight and litter size, respectively.). The h2  R of index units of 0.14 ± 0.02 calculated from divergence agreed with h2  d of 0.14 ± 0.04. Divergences in litter size (-0.19 ± 0.07) and 6-week body weight (0.46 ± 0.10) were in the expected direction. Antagonistic index selection yielded about one-half the expected divergence in litter size, while divergence in 6-week body weight was only slightly less than expected. Realized genetic correlations indicated that litter size, 6-week body weight and index units each showed positive pleiotropy with 3-week body weight, postweaning gain and weight at vaginal introitus and negative pleiotropy with age at vaginal introitus. Sex ratio and several components of fitness (days from joining to parturition, percent fertile matings and percent perinatal survival) did not change significantly in the selected lines.