GENETIC MAP OF THE LACTOSE REPRESSOR GENE (i) OF ESCHERICHIA COLI

Abstract

ABSTRACT 39 tonB i- deletions were used to map 57 independently isolated i gene mutants. Methods selective for i+ recombinants from various types of i mutants are described. i+ recombination frequencies of 6 × 10-6 to 6 × 10-7 can be detected in the different selective systems. Twenty i  s mutations and 12 i  -d mutations map in distinct but different regions of the gene. i  -  sus mutations are scattered over the gene.