A METHOD FOR COMPLEMENTATION ANALYSIS OF NUCLEAR AND CHLOROPLAST MUTANTS OF PHOTOSYNTHESIS IN CHLAMYDOMONAS

Author:

Bennoun Pierre1,Masson Arlette1,Delosme Monique1

Affiliation:

1. Institut de Biologie Physico-Chimique, 13, rue Pierre et Marie Curie, 75005 Paris France

Abstract

ABSTRACT The photosynthetic properties of young zygotes of Chlamydomonas reinhardi were analyzed. In heterozygotes for two nuclear or two chloroplast mutations affecting photosynthesis, recovery of photosynthetic activity was observed that is most likely the result of intergenic complementation.—We observed that chloramphenicol inhibited the recovery of activity in double heterozygotes for mutants lacking at least one thylakoid polypeptide of chloroplast origin, while it had no effect on wild-type homozygotes. This indicates that the recovery of activity in double heterozygotes could result from the repair of existing thylakoid membranes by de nouo synthesis of the missing polypeptides.

Publisher

Oxford University Press (OUP)

Subject

Genetics

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