Tip Loci: Six Chlamydomonas Nuclear Suppressors That Permit the Translocation of Proteins with Mutant Thylakoid Signal Sequences

Author:

Bernd Karen K1,Kohorn Bruce D1

Affiliation:

1. Developmental, Cell and Molecular Biology Group, Department of Botany, Duke University, Durham, North Carolina 27708

Abstract

Abstract Mutations within the signal sequence of cytochrome f (cytf) in Chlamydomonas inhibit thylakoid membrane protein translocation and render cells nonphotosynthetic. Twenty-seven suppressors of the mutant signal sequences were selected for their ability to restore photoautotrophic growth and these describe six nuclear loci named tip1 through 6 for thylakoid insertion protein. The tip mutations restore the translocation of cytf and are not allele specific, as they suppress a number of different cytf signal sequence mutations. Tip5 and 2 may act early in cytf translocation, while Tip1, 3, 4, and 6 are engaged later. The tip mutations have no phenotype in the absence of a signal sequence mutation and there is genetic interaction between tip4, and tip5 suggesting an interaction of their encoded proteins. As there is overlap in the energetic, biochemical and genetic requirements for the translocation of nuclear and chloroplast-encoded thylakoid proteins, the tip mutations likely identify components of a general thylakoid protein translocation apparatus.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Cited by 6 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Understanding Photosynthetic Electron Transport Using Chlamydomonas: The Path from Classical Genetics to High Throughput Genomics;Functional Genomics and Evolution of Photosynthetic Systems;2011-08-29

2. References to Volume 1;The Chlamydomonas Sourcebook;2009

3. Beyond Complementation. Map-Based Cloning in Chlamydomonas reinhardtii;Plant Physiology;2005-02-01

4. Chlamydomonas reinhardtii and photosynthesis: genetics to genomics;Current Opinion in Plant Biology;2000-04

5. Characterization of the Arabidopsis PPI1 Mutant;The Chloroplast: From Molecular Biology to Biotechnology;1999

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