A Genetic Analysis of Glucocorticoid Receptor Signaling: Identification and Characterization of Ligand-Effect Modulators in Saccharomyces cerevisiae-Reference-Cited by-同舟云学术

A Genetic Analysis of Glucocorticoid Receptor Signaling: Identification and Characterization of Ligand-Effect Modulators in Saccharomyces cerevisiae

Published:2000-11-01 Issue:3 Volume:156 Page:963-972
ISSN:1943-2631
Container-title:Genetics
language:en
Short-container-title:

Author:

Sitcheran Raquel¹,Emter Roger²,Kralli Anastasia²,Yamamoto Keith R¹

Affiliation:

1. Department of Cellular and Molecular Pharmacology, University of California, San Francisco, California 91413-0450

2. Division of Biochemistry, Biozentrum, University of Basel, Basel CH4056, Switzerland

Abstract

Abstract To find novel components in the glucocorticoid signal transduction pathway, we performed a yeast genetic screen to identify ligand-effect modulators (LEMs), proteins that modulate the cellular response to hormone. We isolated several mutants that conferred increased glucocorticoid receptor (GR) activity in response to dexamethasone and analyzed two of them in detail. These studies identify two genes, LEM3 and LEM4, which correspond to YNL323w and ERG6, respectively. LEM3 is a putative transmembrane protein of unknown function, and ERG6 is a methyltransferase in the ergosterol biosynthetic pathway. Analysis of null mutants indicates that LEM3 and ERG6 act at different steps in the GR signal transduction pathway.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Link

https://academic.oup.com/genetics/article-pdf/156/3/963/42032122/genetics0963.pdf

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