GENETIC ANALYSIS OF MULTIPLE DRUG CROSS RESISTANCE IN SACCHAROMYCES CEREVISIAE: A NUCLEAR-MITOCHONDRIAL GENE INTERACTION

Author:

Cohen Joe D1,Eaton Norman R1

Affiliation:

1. Department of Biology, Brooklyn College, Brooklyn, New York

Abstract

ABSTRACT A mutant of the yeast Saccharomyces cereuisiae, cross resistant to several antibiotics, was isolated in our laboratory and subjected to genetic analysis. Tetrad analysis of diploids obtained from crosses between the resistant mutant and a sensitive wild-type strain suggest that the multiple resistance to the five agents, oligomycin (OLI), rhodamine 6G (RHG), tetracycline (TCN), chloramphenicol (CAP) and cycloheximide (CHX) is determined by a single nuclear gene, anti, and requires several cytoplasmic genes for expression of resistance to oligomycin, rhodamine 6G and tetracycline.——Vegetatively growing diploid clones derived from the cross ant1 [RHO+] × + [RHO+] show mitotic segregation of two phenotypic classes for the drugs OLI, RHG TCN. Diploids derived from the two reciprocal crosses, ant1 [RHO+] × + [RHO-] and ant1 [RHO-] × + [RHO+], fail to exhibit mitotic segregation. These results are consistent with our hypothesis concerning the involvement of cytoplasmic loci. They suggest, in addition, that these loci are associated with mitochondrial DNA (mtDNA) .——Evidence for this association is provided by the demonstration of genetic linkage between the cytoplasmic loci involved in the interaction, RHG-1, TCN-1 and OLI-5, and two well-characterized mitochondrial loci, ERY and CAP.——We have mapped the nuclear ant1 locus 3.3 cM from the centromere-linked gene, leu1, on the same side of the centromere of chromosome VII as leu1.——In the light of these findings, we discuss the claims made by several authors of the episomal nature of mutations similar ta the one described here, as well as of the possible involvement of yeast 2μ DNA in such mutations.

Publisher

Oxford University Press (OUP)

Subject

Genetics

Cited by 15 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Multiple or pleiotropic drug resistance in yeast;Biochimica et Biophysica Acta (BBA) - General Subjects;1991-03

2. Structure and function of proton translocating ATPase in plasma membranes of plants and fungi;Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes;1988-02

3. The multidrug resistance gene PDR1 from Saccharomyces cerevisiae.;Journal of Biological Chemistry;1987-12

4. Genetic mapping of nuclear mucidin resistance mutations in Saccharomyces cerevisiae;Current Genetics;1986-05

5. Human cell variants resistant to rhodamine 6G;Somatic Cell and Molecular Genetics;1985-11

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3