Mapping of Drosophila Mutations Using Site-Specific Male Recombination

Author:

Chen Bin1,Chu Tehyen12,Harms Emily13,Gergen J Peter342,Strickland Sidney132

Affiliation:

1. Department of Pharmacology, University at Stony Brook, Stony Brook, New York 11794-8651

2. Program in Molecular and Cellular Biology, University at Stony Brook, Stony Brook, New York 11794-8651

3. Program in Genetics, Institute for Cell and Developmental Biology, University at Stony Brook, Stony Brook, New York 11794-8651

4. Department of Biochemistry and Cell Biology, University at Stony Brook, Stony Brook, New York 11794-8651

Abstract

Abstract Although recombination does not usually occur in the male Drosophila germline, site-specific recombination can be induced at the ends of P elements. This finding suggested that male recombination could be used to map Drosophila mutations. In this article, we describe the general method and its application to the mapping of two EMS-induced female-sterile mutations, grauzone and cortex. Within two months, the grauzone gene was mapped relative to seven different P-element insertion sites, and cortex was mapped relative to 23 different P-elements. The results allowed us to map grauzone to a region of about 50 kb, and cortex distal to the chromosomal region 33E. These experiments demonstrate that P-element-induced site-specific male recombination is an efficient and general method to map Drosophila autosomal mutations.

Publisher

Oxford University Press (OUP)

Subject

Genetics

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